Leeds United first team player returns from injury as Whites youngsters face Arsenal
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Shackleton has recently recovered from an Achilles problem and was with Jesse Marsch's first team squad for Monday night's Premier League clash at Crystal Palace but did not make the bench.
The 24-year-old has not featured since coming on as a second-half substitute in February's 4-0 defeat at home to Tottenham Hotspur but Shackleton now starts Friday's hugely important game for the 23s against the Gunners.
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Hide AdKeeper Dani Van den Heuvel, Leo Hjelde, Jeremiah Mullen and Stuart McKinstry also all come into the side for the 7pm kick-off at the Emirates Stadium whilst 16-year-old Archie Gray once again starts.
The young Whites sit third-bottom in the Premier League Two Division One table and just one point ahead of the drop zone.
Second-bottom Chelsea also have two games left and the young Blues will take in both of their remaining fixtures after United's campaign is finished.
However, Leeds are just three points behind fifth-bottom Everton who have a minus nine worse goal difference and the young Toffees are away at champions Manchester City tonight in another 7pm kick-off.
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Hide AdUnited could consequently seal their top-flight status with a victory should Everton get beat although Arsenal are riding high in third place.
Leeds United under-23s v Arsenal: Van den Heuvel, Shackleton, Hjelde, Kenneh, Mullen, Jenkins, McGurk, Bate, Joseph, Gray, McKinstry. Subs: Ombang, Sutcliffe, Ferguson, Miller, Allen.